NAME  ___________________________________________________   LAB  DAY________________________         Lab  Exercise  3:    Tissues                                                                            DATE____________________________      

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Identify  the  following  structures  while  reviewing  Animation,  Dissection,  Histology,  and  models.     All  questions  on  lab  quizzes  and  practicals  will  come  from  the  objectives  below.    Use  Anatomy  &   Physiology  Revealed  and  models  to  help  find  the  structures  first  by  yourself  or  working  with   another  student.    If  unsuccessful,  then  ask  the  lab  instructor  to  guide  you.    

Animation:   Epithelial  tissue  overview   Connective  tissue  overview   Muscle  tissue  overview   Nervous  tissue  overview     Histology:   Epithelial  tissue  -­  Simple     Simple  squamous  epithelium   Nucleus  of  squamous  cell   Simple  squamous  epithelium   Squamous  cell     Simple  cuboidal  epithelium   Cuboidal  epithelial  cell   Nucleus  of  cuboidal  epithelial   cell   Simple  cuboidal  epithelium   (tangential  section)   Lumen  of  collecting  duct   Simple  cuboidal  epithelium     Simple  columnar  epithelium   (ciliated)   Cilia  on  simple  columnar   epithelium   Lumen  of  uterine  tube   Lamina  propria  of  simple   columnar  epithelium   (ciliated)   Simple  columnar  epithelium   (ciliated)     Simple  columnar  epithelium   (nonciliated)   Basement  membrane  of   simple  columnar  epithelium   Columnar  epithelial  cell   (nonciliated)   Goblet  cell  in  nonciliated   simple  columnar  epithelium  

Lamina  propria  of  simple   columnar  epithelium   (nonciliated)   Lumen  of  jejunum   Microvilli  (brush  border)   Nucleus  of  columnar   epithelial  cell  (nonciliated)   Simple  columnar  epithelium   (nonciliated)     Pseudostratified  columnar   epithelium   Basal  body   Cilia  on  pseudostratified   columnar  epithelium   Columnar  epithelial  cell   (ciliated)   Goblet  cell  in  pseudostratified   columnar  epithelium   Lamina  propria  of   pseudostratified  columnar   epithelium   Lumen  of  trachea   Nucleus  of  basal  cell   Pseudostratified  columnar   epithelium     Epithelial  tissue  -­  Stratified     Stratified  squamous   epithelium  (keratinized)   Dermis   Epidermis   Squamous  cell  (without   nucleus)   Stratified  squamous   epithelium  (keratinized)   Stratum  basale   Stratum  corneum   Stratum  granulosum   Stratum  spinosum    

Stratified  squamous   epithelium  (nonkeratinized)   Lamina  propria  of  stratified   squamous  epithelium   Squamous  cell  (with  nucleus)   Stratified  squamous   epithelium  (nonkeratinized)     Stratified  cuboidal  epithelium   Lumen  of  interlobular  duct  of   parotid  gland   Stratified  cuboidal  epithelium   Venule     Stratified  columnar  epithelium   Basement  membrane  of   stratified  columnar   epithelium   Lamina  propria  of  stratified   columnar  epithelium   Stratified  columnar   epithelium   Columnar  epithelial  cell   Lumen  of  spongy  urethra     Transitional  epithelium   Dome-­‐shaped  luminal  cells  of   transitional  epithelium   Lumen  of  allontoic  duct   Loose  connective  tissue   Transitional  epithelium     Connective  tissue  proper  –   loose     Areolar  connective  tissue   Capillary   Ground  substance  of  areolar   connective  tissue   Nucleus  of  fibroblast   Collagen  fiber  in  areolar   connective  tissue  

NAME  ___________________________________________________   LAB  DAY________________________         Lab  Exercise  3:    Tissues                                                                            DATE____________________________      

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Mast  cell  and  granules   Elastic  fiber  in  areolar   connective  tissue   Nucleus  of  endothelial  cell     Adipose  connective  tissue   Adipocyte   Lipid  inclusion  in  adipocyte   Nucleus  of  adipocyte     Reticular  connective  tissue   Ground  substance  of   recticular  connective  tissue   Reticular  fiber     Connective  tissue  proper  –   dense     Dense  regular  connective   tissue   Collagen  fiber  in  dense   regular  connective  tissue   Ground  substance  of  dense   regular  connective  tissue     Dense  irregular  connective   tissue   Collagen  fiber  in  dense   irregular  connective  tissue   Ground  substance  of  dense   irregular  connective  tissue     Elastic  connective  tissue   Elastic  lamellae   Lumen  of  elastic  a.   Tunica  media  of  elastic  a.   Endothelium   Nucleus  of  smooth  muscle  cell   Internal  elastic  lamella    

Supporting  connective   tissue  –  cartilage   Hyaline  cartilage   Chondrocyte  (in  lacuna)  in   hyaline  cartiage   Lacuna  (with  chondrocyte)  in   hyaline  cartilage   Perichrondium  of  hyaline   cartilage   Extracellular  matrix  of  hyaline   cartilage   Nucleus  of  chondrocyte     Fibrocartilage   Chondrocyte  (in  lacuna)  in   fibrocartilage   Ground  substance  of   fibrocartilage   Collagen  fiber  in  fibrocartilage   Lacuna  (with  chondrocyte)  in   fibrocartilage     Elastic  cartilage   Chondrocyte  (in  lacuna)  in   elastic  cartilage   Elastic  fiber  in  elastic   cartilage   Ground  substance  of  elastic   cartilage   Lacuna  (with  chondrocyte)  in   elastic  cartilage   Perichondrium  of  elastic   cartilage     Supporting  connective   tissue  –  bone     Compact  bone   Interstitial  lamella   Osteon   Osteocyte  in  lacuna  

  Spongy  bone   Marrow  cavity  in  spongy  bone   Trabecula  of  spongy  bone     Fluid  connective  tissue     Blood   Erythrocyte   Leukocyte   Platelet     Muscle  tissue     Skeletal  muscle   A  band   I  band   Nucleus  of  skeletal  muscle   fiber     Cardiac  muscle   Branched  cardiac  muscle  fiber   Intercalated  disc   Nucleus  of  cardiac  muscle   fiber     Smooth  muscle   Longitudinal  smooth  muscle   fiber   Nucleus  of  smooth  muscle   fiber   Smooth  muscle  fiber     Nervous  tissue   Nervous  tissue   Axon   Dendrite   Neuron   Nucleolus  in  nucleus  of   neuron   Nucleus  of  glial  cell   Nucleus  of  neuron  

     

 

 

 

 

 

  Total  Structures:    113    

NAME  ___________________________________________________   LAB  DAY________________________         Lab  Exercise  3:    Tissues                                                                            DATE____________________________      

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Station  1:    Anatomy  &  Physiology  Revealed  

Module  3:    Tissues   Make  Sure  to  CLICK  TAGS  ‘ON’  for  Histology  Views  

A. Animation:    Epithelial  Tissue  Overview   1. What  does  epithelium  mean?  _______________________________________________________________________   2. What  is  the  purpose  for  your  body  to  have  epithelium?  _________________________________________   3. How  are  epithelial  cells  classified?  _________________________________________________________________   4. What  is  the  difference  between  simple  and  stratified?  ___________________________________________   5. Where  might  you  find  keratinized  stratified  epithelium?  ________________________________________   6. Which  part  of  the  epithelial  cell  will  increase  absorption?  _______________________________________   7. What  are  the  four  types  of  intercellular  junctions?  _______________________________________________   8. What  are  connexons?  ________________________________________________________________________________  

  B. Histology:    Epithelial  tissue  –  simple  squamous  

1. What  does  ‘simple  squamous’  mean?  ______________________________________________________________   2. Does  the  term  ‘simple  stratified’  exist?  Why  or  why  not?  ________________________________________   3. Name  the  locations  where  you  will  find  simple  cuboidal?  _______________________________________   4. What  is  the  difference  between  a  cuboidal  and  a  columnar  cell?  ________________________________   5. What  is  the  function  of  the  cilia  that  is  attached  to  the  columnar  cell?  __________________________   6. Draw  an  example  of  each  cell:  

  Simple  Squamous                Stratified  Cuboidal                  Simple  Columnar                      

C. Histology:    Epithelial  tissue  –  stratified  squamous   1. What  is  the  function  of  the  keratinized  squamous  layer?  ________________________________________   2. Name  a  location  where  you  will  find  keratinized  squamous  cells.  ______________________________    

D. Animation:    Connective  Tissue  Overview   1. What  two  substances  that  makes  the  matrix?  _____________________________________________________   2. Difference  between  loose  and  dense  connective  tissue?  _________________________________________   3. Areolar  tissue  is  a  subcategory  of  what  type  of  connective  tissue?  ______________________________   4. Dense  regular  tissue  help  form  _____________________________________________________________________   5. Where  might  you  find  hyaline  cartilage?  __________________________________________________________    

E. Histology:    Connective  tissue  proper  –  loose   1. What  is  the  function  of  elastic  fiber  in  areolar  tissue?  ____________________________________________   2. What  type  of  cells  are  adipocytes?  _________________________________________________________________   3. True  or  False:    Adipose  tissues  contain  a  peripherally-­‐located  nucleus  in  their  cells.  _________  

NAME  ___________________________________________________   LAB  DAY________________________         Lab  Exercise  3:    Tissues                                                                            DATE____________________________      

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4. True  or  False:    Reticular  tissue  is  greatly  involved  in  the  lymphatic  system.  ___________________    

F. Histology:    Connective  tissue  proper  –  dense   1. True  or  False:    Dense  tissue  has  more  ground  substance  than  loose.  ___________________________   2. Why  is  dense  regular  tissue  used  for  tendons  and  ligaments?  ___________________________________   3. How  does  the  arrangement  of  fibers  differ  in  dense  regular  vs.  dense  irregular  tissue?  ______  

_________________________________________________________________________________________________________   4. What  is  the  function  of  elastic  tissue  in  the  tunica  media  of  an  elastic  artery?  _________________   5. Draw  the  following  

Dense  regular                                            Dense  irregular                                            Adipose  Tissue    

G. Histology:    Supporting  connective  tissue  –  cartilage   1. What  is  the  purpose  of  a  lacuna?  ___________________________________________________________________   2. What  type  of  cell  is  a  chondrocyte?  ________________________________________________________________    

H. Histology:    Supporting  connective  tissue  –  bone   1. What  is  an  osteocyte?  _______________________________________________________________________________   2. What  is  the  structural  unit  of  bone?  ________________________________________________________________   3. True  or  False:    Compact  bone  is  the  outer  portion  and  the  spongy  bone  is  inner  ______________    

I. Histology:    Fluid  connective  tissue  –  blood   1. What  type  of  cell  has  the  kidney  shaped  nuclei?  __________________________________________________   2. Red  blood  cells  are  also  known  as  __________________________________________________________________   3. Why  do  you  think  the  center  of  the  erythrocyte  is  light  in  color?  _______________________________   4. What  is  the  function  of  platelets?  __________________________________________________________________   5. What  is  the  function  of  white  blood  cells?  _________________________________________________________    

J. Animation:    Muscle  Tissue  Overview   1. What  type  of  fibers  form  muscle  tissue?  ___________________________________________________________   2. Which  type  of  muscle  tissue  is  usually  attached  to  bones?  _______________________________________   3. Describe  the  perimysium.  ___________________________________________________________________________   4. Each  myofibril  is  composed  of  what  type  of  bundles?  ____________________________________________   5. What  type  of  tissue  forms  a  covering  around  the  muscle  fibers?  ________________________________   6. What  organ  is  cardiac  muscle  is  found  in?  _________________________________________________________   7. True  or  False:    Intercalated  discs  are  a  unique  feature  to  all  muscle  tissue.  ____________________   8. Where  is  smooth  muscle  tissue  located?  __________________________________________________________   9. Describe  the  shape  of  a  smooth  muscle  cell.  ______________________________________________________    

NAME  ___________________________________________________   LAB  DAY________________________         Lab  Exercise  3:    Tissues                                                                            DATE____________________________      

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K. Histology:    Muscle  tissue  –  skeletal  muscle   1. Describe  what  the  dark  band  represents.  _________________________________________________________   2. Describe  what  the  light  band  represents.  __________________________________________________________    

L. Histology:    Muscle  tissue  –  cardiac  muscle   1. What  is  the  function  of  an  intercalated  disc?  ______________________________________________________  

    2.   Draw  the  following     Skeletal  Muscle             Cardiac  Muscle  

         

M. Histology:    Muscle  tissue  –  smooth  muscle   1. Smooth  muscle  fiber  is  also  known  as  _____________________________________________________________    

N. Animation:    Nervous  Tissue  Overview   1. What  are  the  two  types  of  cells  that  compose  nervous  tissue?  __________________________________   2. What  is  the  purpose  of  an  axon?  ___________________________________________________________________   3. What  are  the  four  types  of  glial  cells?  ______________________________________________________________   4. True  or  False:    Schwann  cells  are  found  in  the  CNS.  ______________________________________________   5. Describe  the  blood-­‐brain  barrier.  __________________________________________________________________   6. Which  glial  cell  plays  an  important  role  in  the  production  and  circulation  of  CSF?  ____________   7. When  are  microglia  activated?  _____________________________________________________________________   8. Which  type  of  PNS  cell  myelinate  the  axons?  ______________________________________________________    

O. Histology:    Nervous  tissue  -­  nervous   1. Draw  a  neuron     Label  the  axon,  cell  body,  and  dendrite  

 

 

 

 

 

 

NAME  ___________________________________________________   LAB  DAY________________________         Lab  Exercise  3:    Tissues                                                                            DATE____________________________      

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P. Fill  in  the  tables   Type  of  Epithelium   Describe  structure   Location   Function  

  One  layer  of  flattened  cell      

    epidermis    

    Urinary  bladder    

  One  layer  cube     Glandular  secretion  

Simple  columnar        

      Protection/abrasion  

     

Type  of  Tissue   Location   Function  

    Stores  fat  

  Tendons  and  ligaments   Anchors  muscles     IVD   Shock  absorption   Bone      

Areolar  connective         Articular  cartilage,  synovial  joints    

     

Type  of  Muscle   Voluntary/Involuntary   Location   Function  

  Voluntary      

    Heart    

      Internal  organ  function  

 

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